The objective of this study was to investigate the effect of ingestion of powder of hemp seed on blood picture. This study was experienced on five guinea pigs that fed with normal diet (fresh vegetable and tab water), in addition were force-fed 5 g/kg/day of the powder of hemp seed for 60 days by means of an endogastric tube and syringe. At the beginning day and the termination of study day-60 the blood was taken from animals and the erythrocyte number, leukocyte number, packed cell volume (PCV), and hemoglobin concentration values were determined. The result on analysis showed that erythrocyte-count and PCV significantly decreased (p < 0.05) whereas hemoglobin concentration and leukocyte number values showed a steady decline which was not significant (P > 0.05). None of the values fell below the normal physiological range of the experimental animals. This shows that hemp seed which contains tetrahydrocannabinols as its active constituents has long term significant toxicological implication such as bone marrow suppression with respect to the concentration given on the erythrocytes of mammals. It is recommended that individuals who have anemia or immunity complication should not use hemp seed in their food preparation on regular basis.
Our data suggested that chronic use of cannabinoids can lead to deterioration of hematopoietic cells. Chronic use of cannabinoids was consistent with subthreshold/subclinical megaloblastic anemia with iron deficiency. Inflammatory cells, especially neutrophil and monocyte counts were higher in SG compared to CG . Thus, recovery of subclinical hematological parameters should be considered in cannabis use disorder patients.
Some effects such as inflammatory disorders may impair function or synthesis processes resulting in some changes on the cell morphology. For example, hemocytometer parameters like red blood cell (RBC) count, mean red blood cell volume (MCV), and red blood cell distribution width (RDW) are frequently used for anemia diagnosis.21, 22 Previous studies have showed that RDW is an important diagnostic and prognostic predictor of various non‐hematologic diseases such as cardiovascular and metabolic diseases,23, 24, 25 autoimmune disease26, and cancer.27 However, the exact mechanisms underlying the association between RDW and these diseases remain unknown.
2. Materials and Methods
Erythrocyte series and serum iron profiles’ characteristics of SG and CG groups and inflammatory series and platelets’ characteristics of both groups are given in Tables 1 and and2 2 .